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How the Jule Gradient Formers work in automatic pumping mode:

The Jule Gradient Former can be automatically operated by connecting it to a peristaltic pump.

 
Step 1: Remove pistons.
 Gradient Former in automatic pumping mode, Step 1 
 Step 2. Fill each cylinder with fluids to be mixed.
                             Gradient Former in automatic pumping mode, Step 2
 Step 3: Insert pistons, start magnetic stirrer and pump.
                                                         Gradient Former in automatic pumping mode, Step 3


As the pump draws liquid out of the Gradient Former, a vacuum is created within the Gradient Former and the  dual pistons follow the liquid downward, keeping the fluid levels equal in each cylinder.  By keeping the fluid levels equal in each chamber a near perfect linear gradient is formed.  Unlike gravity flow type gradient formers, air bubbles cannot stop the flow. 
MANUAL OPERATION OF GRADIENT FORMERS
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