Jule Snap-Gels(TM) for 2D Electrophoresis

For the Ettan Dalt Six/Twelve, Bio Rad Protean Plus Dodeca Cell, Hoefer SE900 and Iso Dalt*

           

Available with Standard and Low Fluorescence Glass

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Example of using the GE Cy Dyes to label proteins.                    

Sample labeled with 2C3 dye Sample labeled with 2Cy2 dye Sample labelec with 2Cy5 Dye See more gel photos: Click here!
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The 3-color image above represents three different human kidney samples, which were loaded onto a single 10-20% gradient Jule Precast Gel.  Each sample was labeled with a different fluorescent dye, then combined and loaded onto the same gel where the spot patterns overlapped perfectly.  The gray images are the images for the three dyes from the same gel.  Tip of the day: Both high and low molecular weight proteins are better resolved when the Ettan Dalt six and Ettan Dalt twelve buffer tank temperature is set at 25 degrees C (not 15 degrees C)   Remove any liquid from top of gels before securing IPG strip in place with agarose (blot with filter paper, if necessary, but do not let top of gel become dry).

4 STEPS TO SELECT GELS

1) Choose Gel Concentration

2) Build Gel Part Number

3) Gel Prices

4) Place an Order

 

Specifications:

- Low fluorescence glass plates or Regular Glass Plates (lower price)

- 1.0 mm gel thickness.  Also available in 1.5mm thickness.

- Front Plate is 21.2 cm tall x 27.6 cm wide and the  Back Plate is 21.7 cm tall x 27.6 wide.

- Gel is 25.5 cm wide.

- Ultra flat top for 2D electrophoresis, poured 1.0 cm from top of front plate.

- 38:1 Acrylamide:Bis ratio

- Fits into the Ettan Dalt Six and Twelve, BioRad Protean Plus Dodeca Cell System, Hoefer SE900, Iso-Dalt.

- Two month shelf life.

Options available:

- Single or Gradient acrylamide concentrations (you specify).

- Bind Silane can be applied to one of the glass plates. Gel adheres to the glass plate which contains Bind Silane.  Ideal for spot picking. There is a $5.00 charge per Gel for Bind Silane.  Please specify whether to apply Bind Silane to the tall glass plate or the short glass plate when ordering. Jule, Inc. has greatly improved the adherence of Bind Silane.

Repel Silane can be applied to opposite plate upon request (no extra charge). Repel Silane enables removal of glass plate easier.

- Spot Picker Reference Markers (2 markers) can be attached to the glass plate containing Bind Silane. See Markers (circular dots) that were applied the glass plate/Gel (below) at the left and right edges, near the spacers. There is no extra charge for the 2 Markers. The Markers help to establish reference points for spot picking.

- 19:1 Acrylamide:Bis Ratio (38:1 is standard).

- Standard glass (less expensive). See Gel Prices, step 3:

-  Sold in packs of 2 or more gels. You may purchase any quantity needed.  

 

 

                                                        

 

 

 

- Gel Buffer is typically Tris-HCl for the Tris-Glycine System. More about Buffer System Selection

The above Jule Snap-A-Gel Cassettes will fit and run in the equipment shown below.
Ettan Dalt SIX Ettan Dalt Twelve Hoefer SE900  Iso Dalt (Hoefer/GE)
Ettan Dalt Six Ettan Dalt Twelve Hoefer SE 900

Iso-Dalt (Hoefer-GE)

Iso-Dalt users should request enlarged spacer

 

Below are some items which can help you in your work (click on image to learn more).

Large Containers

Buffers

Standards

   
Gel lifting and handling tool kit Buffers, running and sample Protein Standard