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Jule Precast Gels

Here is a sampling of published research by users of Jule, Inc Precast Gels!

Click on link: 

http://scholar.google.com/scholar?q=Jule%2C+Inc.+milford+ct+proteins+biomarker&btnG=&hl=en&as_sdt=0%2C7


 

Some samples listed below.  (Click on above Link for better results)

Proteomic Responses of BEAS-2B Cells to Nontoxic and Toxic Chromium:Protein Indicators of Cytotoxicity Conversion

M Bruno, J Ross, Y Ge - Toxicology Letters, 2016 - Elsevier
... To this end, the alterations in protein expression and phosphorylation identified by both ELISA
and 2 ... This approach also led to the identification of 12 proteins that were not previously associated ...
Precast 8-16% gradient polyacrylamide gels were from Jule, Inc (Milford, CT, USA). ...

[HTML] Proteomic Assessment of Biochemical Pathways That Are Critical to Nickel-Induced Toxicity Responses in Human Epithelial Cells

Y Ge, M Bruno, N Haykal-Coates, K Wallace… - PloS one, 2016 - journals.plos.org
... Precast 8–16% gradient polyacrylamide gels were from Jule, Inc (Milford, CT, USA ... protein
expression and phosphorylation changes using multiplexed ELISA and 2-DE analysis of protein
expression and ... Isolation of total proteins from BEAS-2B cells for 2-DE gel electrophoresis. ...






[HTML] Function of Integrin-Linked Kinase in Modulating the Stemness of IL-6–Abundant Breast Cancer Cells by Regulating γ-Secretase–Mediated Notch1 Activation …

EC Hsu, SK Kulp, HL Huang, HJ Tu, SB Salunke… - Neoplasia, 2015 - Elsevier
... separated by electrophoresis on a 4% to 12% gradient Blue Native Gel (Jule, Inc, Milford, CT)
according to ... Caveolin-1–interacting proteins were examined by immunoblot analysis. ... as means ±
SD (n = 3). (D) Dose-dependent suppressive effects of T315 on protein expression of ...

Bacillus anthracis overcomes an amino acid auxotrophy by cleaving host serumproteins

A Terwilliger, MC Swick, KJ Pflughoeft… - Journal of …, 2015 - Am Soc Microbiol
... were fed into the Protein Pilot program (version 4.5; Paragon-based software from AB Sciex Inc.)
for a ... min and stored at −20°C. All SDS-polyacrylamide gels were 16% Tris-Gly (Jule, Milford, CT),
4 μl ... Proteins were eluted with a linear gradient of 50 mM to 1 M NaCl by using an ...

[HTML] Estrogen regulates energy metabolic pathway and upstream AMPK kinase and phosphatase enzyme expression in dorsal vagal complex metabolo-sensory …

P Tamrakar, BA Ibrahim, AD Gujar… - Journal of neuroscience …, 2015 - ncbi.nlm.nih.gov
... 2009]. Cell and tissue samples were separated on 4–12% or 4–20% gradient
Tris-glycine gels (Jule Biotechnologies, Milford, CT) and transferred to 0.45 μm
PVDF-Plus membranes (Osmonics Inc., Gloucester, MA). Membranes ...

[HTML] Comparative Proteomic Analysis of Two Uveitis Models in Lewis RatsProteomic Analysis in Two Rat Models of Uveitis

KL Pepple, L Rotkis, L Wilson, A Sandt… - … & visual science, 2015 - arvojournals.org
... The second dimension was run on precast 4% to 20% Tris-glycine gels in low fluorescence glass
(Jule, Inc., Milford, CT, USA) at a constant current of 24 mA for 6.5 hours at 4°C. Images were
scanned on a ... Group 2 proteins include lacrimal gland protein (9.8 kDa), α2μ ...

Estrogen regulates energy metabolic pathway and upstream adenosine 5′‐monophosphate‐activated protein kinase and phosphatase enzyme expression in dorsal …

P Tamrakar, BA Ibrahim, AD Gujar… - Journal of …, 2015 - Wiley Online Library
... VC 2014 Wiley Periodicals, Inc. Journal of Neuroscience Research 93:321–332 (2015) ... Cell and
tissue samples were separated on 4–12% or 4–20% gradient Tris-glycine gels (Jule Biotechnolo-
gies, Milford, CT) and transferred to 0.45-lm PVDF-Plus 322 Tamrakar et al. ...

[HTML] Proteomic Assessment of Biochemical Pathways That Are Critical to Nickel-Induced Toxicity Responses in Human Epithelial Cells

Y Ge, M Bruno, N Haykal-Coates, K Wallace… - PloS one, 2016 - journals.plos.org
... Precast 8–16% gradient polyacrylamide gels were from Jule, Inc (Milford, CT, USA ... protein
expression and phosphorylation changes using multiplexed ELISA and 2-DE analysis of protein
expression and ... Isolation of total proteins from BEAS-2B cells for 2-DE gel electrophoresis. ...

Discovery of novel DNA gyrase inhibiting spiropyrimidinetriones: benzisoxazole fusion with N-linked oxazolidinone substituents leading to a clinical candidate ( …

GS Basarab, P Doig, V Galullo, G Kern… - Journal of medicinal …, 2015 - ACS Publications
... compound properties proved best toward identifying a candidate drug. Among such
properties are solubility, protein binding, and in vivo clearance in addition to
antibacterial activity. Four compounds (1h, 1o, 1t, and 1u) showed ...

 

Quantitative analysis of protein phosphorylation using two-dimensional difference gel electrophoresis.

Z Deng, S Bu… - Methods in molecular biology (Clifton, NJ), 2012 - Springer

... Page 11. Precast gels that are compatible with 2-D DIGE and Ettan Dalt II system can be obtained
commercially (eg, 
Jule Inc). 8. Equilibrate the focused IPG strips in equilibration buffer first with
0.5% DTT and then with 2% iodoacetamide, each for 15 min (see Note 14). 
...

 

Biomarkers of breast cancer apoptosis induced by chemotherapy and TRAIL

S Leong, MJ McKay… - Journal of Proteome …, 2012 - ACS Publications

... TBP, 2.5% acrylamide) for 15 min. For the second dimension, equilibrated IPG strips were
transferred onto 8-16% Tris-glycine 
precast gels (Jules 
Inc., CT, USA), and electrophoresis was
performed overnight in the dark at 10 °C. For MS identification of low
abundance ...

 

Rapid and direct microRNA quantification by an enzymatic luminescence assay

Y Sun, KJ Gregory, NG Chen… - Analytical Biochemistry, 2012 - Elsevier

... Inorganic pyrophosphatase (PPase) was purchased from Roche Applied Science
(Indianapolis, IN). 
Pre-cast 15% polyacrylamide gels were purchased from Jule
, Inc.
(Milford, CT). SYBR Green II was purchased from Lonza (Rockland, ME).

 

Optimization of Stability, Encapsulation, Release, and Cross-Priming of Tumor Antigen-Containing PLGA Nanoparticles

S Prasad, V Cody, JK Saucier-Sawyer… - Pharmaceutical …, 2012 - Springer

... Samples were run on a sodium dodecyl sulfate poly- acrylamide gel electrophoresis (SDS-PAGE)
mini 
gel system (Pre-cast 8–16% Tris–HCl 10-well, Jule Inc, CT, USA) @ 30–35 mA per gel 
before
being transferred to nitrocellulose membranes (0.2 μm, Invitrogen, Carlsbad, CA

 

Quantitative proteomic analysis of PCSK9 gain of function in human hepatic HuH7 cells

NJ Denis, H Palmer-Smith, F Elisma… - Journal of Proteome …, 2011 - ACS Publications

... 59 60 Page 8. -7- Each protein fraction was separated using Jule custom made 15cm
4-12% Tris-glycine 
precast denaturing gels (Jule, Inc., Milford, CT, USA) and silver
stained. 
Gels 
were first placed in 50% methanol, 2.5% acetic ...

 

Molecular analyses of β-glucosidase diversity and function in soil

R Cañizares, E Benitez… - European Journal of Soil Biology, 2011 - Elsevier

... Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed using
the 
precast gel from Jule (Jule Inc, Milford, USA) with the following characteristics: 1.5 mm
thick, 8%–16% acrylamide gradient, Tris–Glycine 
Gels 
with SDS

 

Biomarker Discovery in Serum from Patients with Carotid Atherosclerosis

TJ DeGraba, GT Hoehn, PA Nyquist… - Cerebrovascular …, 2011 - content.karger.com

... Amersham IPG Immobiline Drystrips (pH 3–10 NL, 24 cm) and 18 cm 4–12% gradient Precast
gels (Jule, Milford, Conn., USA) were used for the 1st and 2nd dimensions. These gels were run
using the Ettan DALT 12 system (Amersham Biosciences, Piscataway, NJ, USA). 
...

 

Quantitative Subcellular Analysis of the Effects of the Enigmatic Protein PCSK9

N Denis - 2011 - ruor.uottawa.ca

... Following histodenz subcellular fractionation, each histodenz fraction was separated using custom
made
15cm 4-12% Tris-glycine 
precast denaturing gels (Jule Inc., Milford, CT) and silver stained.
Gel 
lanes were cut and separated into 15 separate 1cm bands. Bands ...

 

 

 

Mitochondrial complex I protein is related to residual feed intake in beef cattle

MH Ramos - 2011 - mospace.umsystem.edu

... Page 29. 21 Range, Jule Inc., Milford, CT) and bovine mitochondria (Mitosciences, Eugene, OR)
were loaded into a polyacrylamide 
pre-cast gel (Mini-Protean TGX, Bio-Rad, Hercules, CA). Gel
was mounted into a mini vertical unit (mighty small, Hoefer,
Holliston, MA)

 

[HTML] Novel Approaches to Detect Serum Biomarkers for Clinical Response to Interferon-β Treatment in Multiple Sclerosis

KS Gandhi, FC McKay, E Diefenbach, B Crossett… - PloS one, 2010 - dx.plos.org

... All the optimization steps were carried out using 11 cm IPG strips and 4–15% pre-cast SDS-PAGE
gels (Bio-Rad Laboratories, CA, USA) except for method F. Protein ... The SDS PAGE was run by
laying the strips on 8–18% large size (20 cm×20 cm) 
precast gels (Jule Inc., CT, US 
..

 

Mutagenesis and Characterization Studies to Develop Novel Bioluminescent Systems

BR Branchini - 2010 - DTIC Document

... Soluble protein extracts of GST-luciferases grown at 22 °C and 37 °C were prepared
as described above. In duplicate experiments, aliquots of each protein lysate were
resolved by SDS-PAGE (7.5% 
precast gels, JULE, Inc., Milford, CT). 
.

 

[HTML] Inferring predominant pathways in cellular models of breast cancer using limited sample proteomic profiling

Y Kulkarni, V Suarez… - BMC cancer, 2010 - biomedcentral.com

... A second equilibration step was done for another 30 min by replacing the DTT with 2.5%
iodoacetamide. Equilibrated strips were transferred onto 24 cm 12% uniform 
precast
SDS-polyacrylamide 
gels (Jule
, Inc., Milford, CT) poured between non-fluorescent glass plates

 

bullet Liposomes targeted by fusion phage proteins
PK Jayanna, VP Torchilin, VA Petrenko - Nanomedicine: Nanotechnology, Biology, and …, 2009 – Elsevier

... Ten-microliter aliquots of the denatured samples were then loaded onto a 16% Non-Gradient Tris-Tricine gel (Jule Inc., Milford, Connecticut). ... 

bullet HIV-1 infected monocyte-derived macrophages affect the human brain microvascular …
M Ricardo-Dukelow, I Kadiu,
W Rozek, J … - Journal of neuroimmunology, 2007 – Elsevier

... Fisher (Pittsburgh, PA). Gels for Ettan Dalt Six System GE Healthcare were purchased from Jule, Inc. (Milford, CT)…   

bullet Adenoviral transfer of vasoactive intestinal peptide (VIP) gene inhibits rat aortic and …
RC St. Hilaire, PJ Kadowitz, JR Jeter - Peptides, 2009 – Elsevier

 ... Equal quantities of proteins were electrophoresed on 4–20% gradient gels (Jule Inc., Milford, CT, USA); … 

bullet HLA-G is found in lipid rafts and can act as a signaling molecule
M Comiskey, KE Domino, CM Warner - Human immunology, 2007 – Elsevier

... Immediately prior to Western blotting (described below), 2× loading buffer (Jule, Inc., Milford, CT) was added to the samples. … 

bullet Plasma membrane cyclic nucleotide phosphodiesterase 3B (PDE3B) is associated with …
R Nilsson, F Ahmad, K Swärd, U Andersson, … - Cellular signaling, 2006 – Elsevier

 ... pellet was rehomogenized in 1 ml buffer A and layered on top of a linear 10–45% sucrose gradient (11 ml, prepared using J17 Model gradient maker from Jule Inc ...  

bullet Comparison of the proteosomes and antigenicities of secreted and cellular proteins …
D Cho, MT Collins - Clinical and Vaccine Immunology, 2006 - Am Soc Microbiol

 ... The equilibrated strips were loaded onto precast 2-DE gels with exponential gradients of 10 to 20% (wt/vol) acrylamide (Jule, Inc., Milford, CT). ...  

bullet Mitochondrial proteomics of the retinal pigment epithelium at progressive stages of age- …
CL Nordgaard, PP Karunadharma, X Feng, TW … - Investigative Ophthalmology & Visual Science, 2008 – ARVO

 ... Samples were also analyzed on 16 x 16 x 1.5-mm, 8% to 16% gradient gels (Jule Inc., Milford, CT) and stained with flamingo pink fluorescent protein stain...   

bullet Gelsolin is Depleted in Post-Shock Mesenteric Lymph
JR Jordan, EE Moore, SS Damle, P Eckels, … - Journal of Surgical Research, 2007 – Elsevier

... Strips were then applied to a precast 8% to 16% SDS-PAGE gel (Jule Inc., Milford, CT) for electrophoresis at a constant 20 W/gel at 25°C in an Ettan DALT 12 ...  

bullet RPE65 is an iron (II)-dependent isomerohydrolase in the retinoid visual cycle
G Moiseyev, Y Takahashi, Y Chen, S … - Journal of Biological Chemistry, 2006 – ASBMB

 ... gels and buffers. Pre-cast native polyacrylamide gradient gels (4-20%) were purchased from Jule Inc. (Milford, CT). …  

bullet HIV-1 transforms the monocyte plasma membrane proteome
I Kadiu, T Wang, JD Schlautman, L … - Cellular Immunology, 2009 – Elsevier

... The 2D Sample Prep for Membrane Proteins Kit and the 10–20% gradient Tris–Glycine gels were from Jule Inc (Milford, CT) … 

bullet A Comparison of Protein Extraction Methods Suitable for Gel-Based Proteomic Studies of …
M Cilia, T Fish, X Yang, M Mclaughlin, TW … - 2009 - pubmedcentral.nih.gov

... equilibration buffer. The second dimension was carried out using 12% PAGE tris-glycine gels (Jule, Inc, Milford, CT). … 

bullet Redox-reactive membrane vesicles produced by Shewanella
Y Gorby, J Mclean, A Korenevsky, K Rosso, … - Geobiology, 2008 - nanobio.usc.edu

... Cl, 4% SDS, 20% v/v glycerol, 0.2 M dithiothreitol, and 0.02% bromophenol blue, pH 6.8), applied to 12.5% precast acrylamide minigels (Jule, Inc., Milford, CT ...

bullet The mammalian target of rapamycin (mTOR) pathway regulates mitochondrial oxygen …
SM Schieke, D Phillips, JP McCoy Jr, AM … - Journal of Biological Chemistry, 2006 – ASBMB

... loaded onto an Ettan DALT-12 electrophoresis unit (GE Healthcare), and the proteins were separated on a 10-15% SDS-polyacrylamide gel (Jule Inc., Milford, CT ...

bullet Detection of Ovarian Matrix Metalloproteinase mRNAs by In Situ Hybridization.

KL Rosewell, TE Curry Jr - Methods in molecular biology (Clifton, NJ), 2009 – Springer

... 117 Page 4. 11. Snap-A-GelsTM mini cassette, 1.5 mm (Jule, Inc.) 12. Gel Loading Buffer II (Ambion) 13. Kodak Biomax XAR film (Thermo Fisher). 2.3. ...  

bullet Gene targeting reveals the role of Oc90 as the essential organizer of the otoconial organic …
X Zhao, H Yang, EN Yamoah, YW Lundberg - Developmental biology, 2007 – Elsevier

... 46 mg/ml SDS, 0.2 mg/ml bromophenol blue, 20 mg/ml DTT), boiled for 5 min, loaded onto a 4–20% gradient Tris–SDS gel (Jule Inc.), electrophoresed at 200 V ...

bullet Analysis of the zebrafish proteome during embryonic development

MB Lucitt, TS Price, A Pizarro, W Wu, AK … - Molecular & Cellular Proteomics, 2008 – ASBMB

…IPG strips were then placed on top of an 8–16% precast polyacrylamide gel (Jule, Inc., Milford, CT). …

bullet Nuclear factor kappa Β in the dorsal raphe of macaques: an anatomical link for steroids, …
CL Bethea, AP Reddy, LJ Smith - Journal of Psychiatry & Neuroscience, 2006 - pubmedcentral.nih.gov

... Western blotting was performed with Tris–glycine precast 10% SDS gels (16 × 18 cm) with 10 lanes (Jule, Inc., Milford, Conn.), ...  

bullet Nitrated α–Synuclein Immunity Accelerates Degeneration of Nigral Dopaminergic Neurons
EJ Benner, R Banerjee, AD Reynolds, S … - PLoS
ONE, 2008 - pubmedcentral.nih.gov

.. N-α-Syn IP samples were fractionated by large format 16% Tricine SDS-PAGE (Jule Inc., Milford, CT; BIORAD Laboratories, Inc, Los Angeles, CA) at constant ...   

bullet Discovery and validation of protein abundance differences between follicular thyroid …

RT Netea-Maier, SW Hunsucker, BM … - Cancer Research, 2008 – AACR

... Gel electrophoresis was performed on precast 8% to 16% acrylamide gradient gels (Jule, Inc.) as previously described (8). …  

bullet Plasmodium yoelii: experimental evidences for the conserved epitopes between mouse and …

L Xu, X Pei, K Berzins, A Chaudhuri - Experimental parasitology, 2007 – Elsevier

... by specific antibodies. MRSPs were analyzed on a 3–27% precast gradient gel (JULE Inc., Research Drive, Milford, CT). …  

bullet Intraflagellar Transport (IFT) Protein IFT25 Is a Phosphoprotein Component of IFT Complex …
Z Wang, ZC Fan, SM Williamson, H Qin - PLoS
ONE, 2009 - pubmedcentral.nih.gov

... Linear 12 ml 10–25% sucrose density gradients in 1×HMDEK buffer plus protease inhibitors were generated by using the Jule Gradient Former (Jule, Inc. ...  

bullet The interaction of mannose binding lectin (MBL) with mannose containing glycopeptides and …
PI Sealy, B Garner, E Swiatlo, SW Chapman, … - Medical Mycology, 2008 - informahealthcare.com

... of sMBL, hMBL, MN and TP were mixed with equal volumes (7.5 µl) of sample buffer prior to loading into wells of Snap-A-Gel™ precast gels (Jule Inc., USA). ...  

bullet Proteomic profiling of endorepellin angiostatic activity on human endothelial cells

JJ Zoeller, RV Iozzo - Proteome Science, 2008 - proteomesci.com

... containing 2.5% iodoacetamide. IPG strips were then directly applied to 8–18% SDS Tris-Glycine gels (Jule Inc. Biotechnologies), ...  

bullet Two-dimensional fluorescence difference gel electrophoresis for comparative proteomics …
NS Tannu,
SE Hemby - Nature protocols, 2006 - nature.com

... Pre-cast 8–15% gradient SDS-PAGE (2,400 2,000 1 mm; Jule Inc.); Fixing solution (30% (vol/vol) methanol and 7.5% (vol/vol) acetic acid); ...  

bullet A new method for accurate assessment of DNA quality after bisulfite treatment

M Ehrich, S Zoll, S Sur, D van den Boom - Nucleic Acids Research, 2007 - Oxford Univ Press

... Ten microliters of the bisulfite-treated DNA was loaded along with 5 µl of loading dye on a 4–8% TBE PAGE pre-cast gel (Jule Inc., Milford, CT). ...

bullet Identification of a second collagen-like glycoprotein produced by Bacillus anthracis and …

LN Waller, MJ Stump, KF Fox, WM Harley, A … - Journal of bacteriology, 2005 - Am Soc Microbiol

... 5% ß-mercaptoethanol, 0.02% bromophenol blue [Fisher Scientific]), loaded onto each lane of 4 to 15% gradient polyacrylamide gels (Jule, Inc., Milliford, CT ...  

bullet Cytochrome P4501A is induced in endothelial cell lines from the kidney and lung of the …

RA Garrick, BR Woodin, JY Wilson, BL … - Aquatic Toxicology, 2006 – Elsevier

 ... of standards of CYP1A from scup (Stenotomus chrysops) were resolved on a 4–20% acrylamide gradient gel in TRIS–glycine with SDS (Jule Inc., Milford, CT). ...  

bullet Cytosolic proteomic alterations in the nucleus accumbens of cocaine overdose victims

N Tannu, DC Mash, SE Hemby - Molecular psychiatry, 2006 - nature.com

... on the basis of their molecular weight on a pre-cast 8–15% gradient SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (2400 2000 1 mm, Jule Inc., Milford, CT ...

bullet Protein adduct-trapping by hydrazinophthalazine drugs: mechanisms of cytoprotection …

PC Burcham, FR Fontaine, LM Kaminskas, DR … - Molecular pharmacology, 2004 – ASPET

... at 4°C. After protein estimation (Pierce BCA Kit), 50 µg of protein was resolved overnight at 4 V/cm on either a 4 to 20% gradient acrylamide gel (Jule Inc. ...  

bullet Immunoproteomic Identification of Secretory and Subcellular Protein Antigens and …

MK Gupta, V Subramanian, JS Yadav - J. Proteome Res, 2009 - pubs.acs.org

... subsequent effort to improve the 2DE-resolution of the secreted proteins, a larger IPG strip (24 cm) and a precast 4−20% gradient gel (Jule, Inc., Milford, CT ...  

bullet Cytochrome P4501A induced differentially in endothelial cells cultured from different organs …

RA Garrick, BR Woodin, JJ Stegeman - In Vitro Cellular & Developmental Biology-Animal, 2005 – Springer

... of CYP1A from scup (Stenotomus chrysops) were resolved on a 4-20% acrylamide gradient gel in TRIS-glycine with sodium dodecyl sulfate (Jule Inc., Milford, CT). ...

bullet Effect of human apolipoprotein E genotype on the pathogenesis of experimental ocular HSV …
PS Bhattacharjee, DM Neumann, TP Foster, S … - Experimental eye research, 2008 – Elsevier

... To quantitate the apoE, 200 μg/well of denatured protein samples were separated on 4%–20% gradient SDS-PAGE (Jule Inc, Milford, CT), ...

bullet YPED: a web-accessible database system for protein expression analysis

MA Shifman, Y Li, CM Colangelo, KL Stone, … - J. Proteome Res, 2007 - pubs.acs.org

... with CyDye DIGE fluor labeling reagents, GE Healthcare Immobiline DryStrip Gels, the Ettan IPGphor IEF System, and pre-poured polyacrylamide gels from Jule Inc ...

bullet Proteomic analysis of a hypochlorous acidtolerant Listeria monocytogenes cultural variant …

JP Folsom, JF Frank - Journal of Food Protection&# 174;, 2007 - ingentaconnect.com

... instructions. IPG strips were loaded onto 1 mM Tris-glycine gra- dient (8 to 15%) gels (Jule, Inc., Milford, Conn.). …

bullet Humic substances and crude oil induce cytochrome P450 1A expression in the Amazonian …
AYO Matsuo, BR Woodin, CM Reddy, AL Val, … - Environ. Sci. Technol, 2006 - pubs.acs.org

... HS-acclimated fish and 2−5 μg from oil-exposed fish) were electrophoretically resolved on 5−20% gradient gels (Precast Snap-A-Gel, Jule Inc.), … 

bullet Cytochrome P450 induced differentially in endothelial cells cultured from different organs of …
RA Garrick, BR Woodin, JJ Stegeman - 2004 - darchive.mblwhoilibrary.org

... CYP1A from scup (Stenotomus chrysops) were resolved on a 4-20 % acrylamide gradient gel in TRIS-glycine with SDS (Jule,Inc, Milford, CT)...  

bullet WUSCHEL is a primary target for transcriptional regulation by SPLAYED in dynamic control of …
CS Kwon, C Chen, D Wagner - Genes & development, 2005 - genesdev.cshlp.org

... et al. 1988) were dissolved in 1× Laemmli SDS sample buffer and run on 4%-12% gradient gel (Jule, Inc.)...  

bullet Immortalization and characterization of lineage-restricted neuronal progenitor cells derived …

AU Uebing-Czipura, HD Dawson, G Scherba - Journal of neuroscience methods, 2008 – Elsevier

... TPG) for each sample, the amplicons were separated during vertical electrophoresis in a non-denaturing 12% polyacrylamide gel (Jule, Inc., Milford, CT) ...  

bullet The oligomerization state determines regulatory properties and inhibitor sensitivity of type …

W Richter, M Conti - Journal of Biological Chemistry, 2004 – ASBMB

... and 5 mM -mercaptoethanol were prepared in 14 x 89-mm centrifugation tubes (Beckman Instruments, Fullerton, CA) using a gradient former (Jule Inc., Milford, CT ...  

bullet MicroRNA–target pairs in the rat kidney identified by microRNA microarray, proteomic, and …

Z Tian, AS Greene, JL Pietrusz, IR Matus, M … - Genome research, 2008 - 171.66.122.45

 ... buffer containing 0.5% DTT, then 15 min in the same buffer with 4.5% (w/v) iodoacetamide, and then loaded onto 12.5% vertical SDS-PAGE slab gels (Jule Inc.). ...
 

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